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Image Search Results
Journal: Scientific Reports
Article Title: Comparison of the sensitivity of Western blotting between PVDF and NC membranes
doi: 10.1038/s41598-021-91521-8
Figure Lengend Snippet: Antibodies, lectins and proteins, as well as their molecular weights, transfer time and incubate time.
Article Snippet: Rabbit anti-ceruloplasmin (CerP) antibody, rabbit anti-transferrin (TF) antibody and
Techniques: Molecular Weight, High Molecular Weight
Journal: Scientific Reports
Article Title: Comparison of the sensitivity of Western blotting between PVDF and NC membranes
doi: 10.1038/s41598-021-91521-8
Figure Lengend Snippet: Comparison of the re-probed ability of PVDF membrane and NC membrane. The pooled sera proteins (3.0, 1.5, 0.7, 0.3 and 0.1 μg) were separated by 8% SDS-PAGE, and transferred to PVDF membranes (up) and NC membrane (down), respectively. ( a ) Staining with AAL and then re-probed with PHA-E; ( b ) staining with ApoA1 and then re-probing with IgG; ( c ) Staining with PHA-E and then re-probing with A2M; ( d ) staining with A2M and then re-probing with PHA-E. Band intensities were statistically analyzed (n = 3 individual experiments) and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed *Significantly different p < 0.05, ** p < 0.01. N.S., not significant. All values are means ± S.E. (error bars).
Article Snippet: Rabbit anti-ceruloplasmin (CerP) antibody, rabbit anti-transferrin (TF) antibody and
Techniques: Comparison, Membrane, SDS Page, Staining, Software